Jackson Estuarine Laboratory

Identification and localization of catecholamines in the nervous system of Limulus polyphemus

Abstract

The concentrations of various catecholamines in the nervous system of the horseshoe crab Limulus polyphemus have been determined by high-performance liquid chromatography with electrochemical detection. Dopamine, norepinephrine, epinephrine, and their precursor L-Dopa were present in appreciable quantities in discrete regions of the central nervous system and cardiac ganglion. The catecholamines were localized more precisely by use of the glyoxylic-acid-histofluorescence technique of de la Torre and Surgeon (1976). Catecholamine fluorescence appeared in protocerebral and tritocerebral neuropile, including regions of the central body and optic medulla. Posterior to these brain areas, tracts extended through the circumesophageal ganglionic ring and laterally out each of the pedal ganglia. Small clusters of large fluorescent somata were present in the protocerebrum. No fluorescence was observed in the corpora pedunculata.

Department

Jackson Estuarine Laboratory, Biological Sciences

Publication Date

1-1-1982

Journal Title

Journal of Neurobiology

Publisher

Wiley

Digital Object Identifier (DOI)

https://doi.org/10.1002/neu.480130106

Document Type

Article

Rights

© 1982 John Wiley & Sons, Inc.

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